Fundamental analysis of recombinant human epidermal growth factor in solution with biophysical methods

Correlation of thermodynamic and secondary structural stability of proteins at various buffer pHs was investigated using differential scanning calorimetry (DSC), dynamic light scattering (DLS) and attenuated total reflection Fourier-transform infrared spectroscopy (ATR FT-IR). Recombinant human epithelial growth factor (rhEGF) was selected as a model protein at various pHs and in different buffers, including phosphate, histidine, citrate, HEPES and Tris. Particle size and zeta potential of rhEGF at each selected pH of buffer were observed by DLS. Four factors were used to characterize the biophysical stability of rhEGF in solution: temperature at maximum heat flux (T_m), intermolecular β-sheet contents, zeta size and zeta potential. It was possible to predict the apparent isoelectric point (pI) of rhEGF as 4.43 by plotting pH against zeta potential. When the pH of the rhEGF solution increased or decreased from pI, the absolute zeta potential increased indicating a reduced possibility of protein aggregation, since T_m increased and β-sheet contents decreased. The contents of induced intermolecular β-sheet in Tris and HEPES buffers were the lowest. Thermodynamic stability of rhEGF markedly increased when pH is higher than 6.2 in histidine buffer where T_m of first transition was all above 70?°C. Moreover, rhEGF in Tris buffer was more thermodynamically stable than in HEPES with higher zeta potential. Tris buffer at pH 7.2 was concluded to be the most favorable.     

Classification of quasigroups based on period factors

Edon80 is a stream cipher design that had advanced to the third and last phase of the eSTREAM project. The core of the cipher consists quasigroup string e-transformations and it employs four quasigroups of order 4. The employed quasigroups have influence on the period of the keystream. There are 576 quasigroups of order 4 in total which have different period factors. The four quasigroups used in Edon80 were chosen by numerous computer experiments. In this paper, based on permutation groups, we give a theory criterion for the determination of quasigroups and give a complete classification for the 576 quasigroups of order 4 according to their period factors.     

网络安全威胁因素及其常见网络安全技术分析

社会经济的快速发展促进了科学技术水平的显著提升，社会生产生活各个领域当中对计算机网络的应用程度不断提升，当前，网络已经成为了人们日常生活与工作的重要组成部分。为此，加强对网络安全等因素影响研究就显得格外重要。网络环境关系着用户的使用质量，更加影响人们的工作效率，加强对网络安全技术的研究时解决相关问题的核心内容。本文针对网络安全威胁因素以及常见的网络安全技术进行了简要分析。     

A new semi-empirical wind turbine capacity factor for maximizing annual electricity and hydrogen production

The capacity factor is an important wind turbine parameter which is ratio of average output electrical power to rated electrical power of the wind turbine. Another main factor, the AEP, the annual energy production, can be determined using wind characteristics and wind turbine performance. Lower rated power may lead to higher capacity factor but will reduce the AEP. Therefore, it is important to consider simultaneously both the capacity factor and the AEP in design or selecting a wind turbine. In this work, a new semi-empirical secondary capacity factor is introduced for determining a rated wind speed at which yearly energy and hydrogen production obtain a maximum value. This capacity factor is expressed as ratio of the AEP for wind turbine to yearly wind energy delivered by mean wind speed at the rotor swept area. The methodology is demonstrated using the empirical efficiency curve of Vestas-80 2 MW turbine and the Weibull probability density function. Simultaneous use of the primary and the secondary capacity factors are discussed for maximizing electrical energy and hence hydrogen production for different wind classes and economic feasibility are scrutinized in several wind stations in Kuwait.     

Identification of Wiener systems based on the variable forgetting factor multierror stochastic gradient and the key term separation

The identification of Wiener systems is very difficult because of the output nonlinearity and the parameter product term. To identify the Wiener system, a novel stochastic gradient algorithm based on the multierror and the key term separation is proposed. Firstly, the Wiener system is parameterized as a pseudo-linear model to avoid the products of the parameters. Secondly, a parzen window is used to estimate the probability density function of the error. Thirdly, a stochastic information gradient algorithm with the multierror is adopted to estimate the parameters. The multierror takes the place of the scalar error by the stacked error, which accelerates the algorithm greatly. Fourthly, a variable forgetting factor considering errors is integrated to further speed the algorithm up. Finally, the proposed algorithm is validated by a numerical example and an industrial case. The estimation results indicate that the proposed algorithm can obtain accurate estimates with fast convergence speed.     

Reduction of Ripple Current Loss by Electromagnetic Coupling of Air‐Core Reactors

To reduce the loss due to ripple current in a multiphase current‐reversible chopper, we investigated electromagnetic coupling of an air‐core reactor. We derived the relationship between the amplitude of the ripple current, the duty factor, and the electromagnetic coupling coefficient, and used the results to estimate the effects of electromagnetic coupling in the design of a train energy storage system. We built reactors with electromagnetic coupling coefficients of 0.93 and 0.60. These reactors employed a new winding structure that provides an optimal electromagnetic coupling coefficient. The mass of the former type of reactor was increased by 4.4% over the conventional design, and that of the latter type of reactor was decreased by 17%. Finally, we tested the new reactors. When the chopper employs the former type of reactor and operates with equal‐phase switching and cumulative coupling, the loss due to ripple current is decreased by 11%. When the chopper employs the latter type of reactor and operates with shift‐phase switching and differential coupling, the loss is decreased to 31%. The test showed that the calculated relationships agreed with the measured values.     

双对苯醌的抗氧化活性分析及其固体发酵条件优化

为探索生物活性未知的双对苯醌（2,7-dihydroxy-3,6,9-trimethyl-9H-xanthene-1,4,5,8-tetraone，DTXT）的抗氧化活性，并提高其发酵产量，考察DTXT的还原力以及对超氧阴离子自由基、羟自由基、1,1-二苯基-2-三硝基苯肼（1,1-diphenyl-2-picrylhydrazyl，DPPH）自由基的清除效果，在单因素试验基础上，采用响应面法优化了DTXT产生菌瓶生顶孢霉（Acremonium cavaraeanum）CA022菌株的固体发酵培养基。结果表明：在200 μg/mL质量浓度下，DTXT的还原力与芦丁差异不显著，高于VE和2,6-二叔丁基-4甲基苯酚，对超氧阴离子自由基清除率达到67.00%，对羟自由基清除率达到78.83%，对DPPH自由基清除率达到76.53%。通过响应面试验，得到最佳培养基配方为葡萄糖0.773%、硝酸钠0.185%、H3BO3 0.032%、VB1 100 μg/100 g，在此条件下实际获得的DTXT产量为4 150.8 mg/kg，是优化前产量的（2 864.83 mg/kg）1.45 倍。     

Structural dimension optimization and mechanical response analysis of fabricated honeycomb plastic pavement slab

Because of favorable mechanical properties, deformation resistance and being conducive to environmental protection, honeycomb fabricated plastic pavement slabs are highly recommended these years. At present, most studies focus on the performance of plastic materials, however, the dimension optimization of fabricated plastic pavement slab is rarely mentioned. In this paper, an optimized geometry of the honeycomb pavement slab was determined through finite element analysis. Mechanical response of honeycomb slabs with different internal dimensions and external dimensions were explored. Several dimension factors were taken into consideration including the side length, rib thickness, the thickness of both top and bottom slabs of honeycomb structure and the length, the width and the thickness of the fabricated plastic slab. The results showed that honeycomb pavement slab with 6 cm bottom slab, 12 cm top slab,18 cm side length and 6 cm rib thickness is recommended, additionally, an external dimension of 4 m × 4 m × 0.45 m is suggested. Then, the mechanical responses of this optimized fabricated plastic slab were further investigated. Significance of different influencing factors, including wheel load, elastic modulus of plastic material, base layer thickness, soil foundation modulus and base layer modulus were ranked.     

剪切解络-超滤分离金属关键指标预测与验证

根据剪切解络-超滤过程中金属离子的动态浓度关系和质量守恒定律,采用CodeBlocks17.12软件编写程序,建立了剪切解络-超滤工艺关键指标预测模型,实现了金属离子浓度、回收率、体积稀释倍数等指标的预测,并用实验加以验证。依次在转速800 r/min、1 400 r/min、3 000 r/min下回收模拟废水中43 μg/L的Co²⁺、995 μg/L的Ni²⁺和88 μg/L的La³⁺,3种金属的回收率均达99.8%以上时,超滤过程体积稀释倍数的预测值分别为7.0、7.5、5.75,实验值分别为7.5、7.25、6.25,可见预测值与实验值基本一致。本模型对研究方案制定和调整有实际指导意义。